Structural and Interaction Analysis of Glycoprotein VI-binding Peptide Selected from a Phage Display Library
نویسندگان
چکیده
منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملScreening of a Specific Peptide Binding to VPAC1 Receptor from a Phage Display Peptide Library
BACKGROUND/PURPOSE The VPAC1 receptor, a member of the vasoactive intestinal peptide receptors (VIPRs), is overexpressed in the most frequently occurring malignant tumors and plays a major role in the progression and angiogenesis of a number of malignancies. Recently, phage display has become widely used for many applications, including ligand generation for targeted imaging, drug delivery and ...
متن کاملscreening phage display library for isolation of human colon cancer cell-binding peptide ligands
objective: recently, phage display libraries have received enormous attention for identification and isolation of pharmaceutical molecules with diagnostic and therapeutic properties. peptide libraries are known as one of the most important and widely used types of phage display libraries. in the current study, we aimed to screen the ph.d.tm-7 phage display peptide library through biopanning for...
متن کاملMimotopes of the Vi antigen of Salmonella enterica serovar typhi identified from phage display peptide library.
The capsular polysaccharide Vi antigen (ViCPS) is an essential virulence factor and also a protective antigen of Salmonella enterica serovar Typhi. A random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific monoclonal antibody (MAb) ATVi. Approximately 75% of the phage clones selected in the fourth round...
متن کاملConstruction of a Nanobodies Phage Display Library From an Escherichia coli Immunized Dromedary
Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2009
ISSN: 0021-9258
DOI: 10.1074/jbc.m808563200